# This is an example configuration file for FastQ Screen ############################ ## Bowtie, Bowtie 2 or BWA # ############################ ## If the Bowtie, Bowtie 2 or BWA binary is not in your PATH, you can set ## this value to tell the program where to find your chosen aligner. Uncomment ## the relevant line below and set the appropriate location. Please note, ## this path should INCLUDE the executable filename. BOWTIE /LUSTRE/apps/bioinformatica/bowtie1/bowtie BOWTIE2 /LUSTRE/apps/bioinformatica/bowtie2/bowtie2 #BWA /usr/local/bwa/bwa ############################################ ## Bismark (for bisulfite sequencing only) # ############################################ ## If the Bismark binary is not in your PATH then you can set this value to ## tell the program where to find it. Uncomment the line below and set the ## appropriate location. Please note, this path should INCLUDE the executable ## filename. #BISMARK /usr/local/bin/bismark/bismark ############ ## Threads # ############ ## Genome aligners can be made to run across multiple CPU cores to speed up ## searches. Set this value to the number of cores you want for mapping reads. THREADS 96 ############## ## DATABASES # ############## ## This section enables you to configure multiple genomes databases (aligner index ## files) to search against in your screen. For each genome you need to provide a ## database name (which can't contain spaces) and the location of the aligner index ## files. ## ## The path to the index files SHOULD INCLUDE THE BASENAME of the index, e.g: ## /data/public/Genomes/Human_Bowtie/GRCh37/Homo_sapiens.GRCh37 ## Thus, the index files (Homo_sapiens.GRCh37.1.bt2, Homo_sapiens.GRCh37.2.bt2, etc.) ## are found in a folder named 'GRCh37'. ## ## If, for example, the Bowtie, Bowtie2 and BWA indices of a given genome reside in ## the SAME FOLDER, a SINLGE path may be provided to ALL the of indices. The index ## used will be the one compatible with the chosen aligner (as specified using the ## --aligner flag). ## ## The entries shown below are only suggested examples, you can add as many DATABASE ## sections as required, and you can comment out or remove as many of the existing ## entries as desired. We suggest including genomes and sequences that may be sources ## of contamination either because they where run on your sequencer previously, or may ## have contaminated your sample during the library preparation step. ## Human - sequences available from ## ftp://ftp.ensembl.org/pub/current/fasta/homo_sapiens/dna/ DATABASE Human /home/rgomez/bin/fastq_screen_v0.11.1/Genomes/human/hg38ome/hg38 ## Ecoli- sequence available from esemble accession GCA_000967155.1 ## ftp://ftp.ensemblgenomes.org/pub/bacteria/release-36/fasta/bacteria_91_collection/escherichia_coli/dna/* DATABASE Ecoli /home/rgomez/bin/fastq_screen_v0.11.1/Genomes/Ecoli/Ecoli_U00096.2 ## PhiX - sequence available from Refseq accession NC_001422.1 # https://www.ncbi.nlm.nih.gov/nuccore/9626372/ DATABASE PhiX /home/rgomez/bin/fastq_screen_v0.11.1/Genomes/PhiX/PhiX ## Adapters - sequence derived from the FastQC contaminats file found at: www.bioinformatics.babraham.ac.uk/projects/fastqc DATABASE Univec /home/rgomez/bin/fastq_screen_v0.11.1/Genomes/UniVec/UniVec ### Scerevisiae - genome available from ## ftp://ftp.ensembl.org/pub/release-89/fasta/saccharomyces_cerevisiae/dna DATABASE cerevisiae /home/rgomez/bin/fastq_screen_v0.11.1/Genomes/s_cerevisiae/s_cerevisiae